2009; Cao et al. Read coverage and SNV density in the N2 reference genome and the iteratively corrected CB4856 genome. In the absence of avermectin, the defective glc-1 may lead to a fitness disadvantage (Ghosh et al. This site is intended for use by persons 21 or older only. We have used a variety of resources and methods to produce a draft reference CB4856 genome sequence. Li Y, Alvarez O A, Gutteling E W, Tijsterman M, Fu J et al. Hawaiian Fire Strain Genetics Because its heritage is unknown, we can only link the Hawaiian Fire strain to the Hawaiian lineage by its name. This strain can present THC levels ranging from 15% to 23%, and it also contains 1% CBD, which, together with its terpene profile, give the final touches to the effects it generates. Hawaiian Unicorn is an indica/sativa variety from ThugPug Genetics and can be cultivated indoors (where the plants will need a flowering time of 64 days) and outdoors. The elucidation of sequence variants in CB4856 has occurred in several steps. This abundant THC content, together with its terpene profile, can generate intense effects of a euphoric nature perfect for the afternoon or the end of a long day. Hawaiian Candy exudes sweet, tropical, and creamy scents, with hints of lemon that balance the final scent profile. Kammenga J E, Doroszuk A, Riksen J A, Hazendonk E, Spiridon L et al. History & Genetics Maui Waui, sometimes called Maui Wowie, is a leaning 80% sativa hybrid that was considered a top-of-the-line strain when it first appeared in the 1960s. 808 Genetics' Hawaiian Sweetheart is a THC dominant variety and is/was never available as feminized seeds. 2015). So with this strain, get ready to experience the unique combination of flavors, smells, and therapeutic qualities of one of the best Hawaiian strains. To identify high-quality substitutions within the LASTZ alignments, we required at least three reads and 150 reads with the fraction of reads that disagree with the total reads at the site as <0.2. Thompson O, Edgley M, Strasbourger P, Flibotte S, Ewing B et al. But I just have an eye for spotting Hawaiian genetics! Resulting from the cross between the Cherry Pie and Hawaiian strains, the Hawaiian Pie strain is truly rare. 2012), that were estimated to have arisen 7 106 generations ago. 2012; Volkers et al. 2013) were aligned against both the N2 and CB4856 reference sequences. . Files in the lav output format were converted to psl format using lav2psl (J. Kent, http://hgdownload.cse.ucsc.edu/admin/jksrc.zip) and were chained [creating a sequence of gapless aligned blocks with no overlapping blocks (Kent et al. The 628-amino-acid predicted protein, when evaluated by blastp (Altschul et al. The effects of Hawaiian Snow can help with headaches and poor appetite since it has the peculiarity of inducing what many stoners know as munchies. 2013). Hawaiian Spice, also simply called Spice, is a rare strain of cannabis that comes from crossing two native Hawaii strains. Schwartz S, Kent W J, Smit A, Zhang Z, Baertsch R et al. Just as the name suggests, this strain is a true delight for any cannabis connoisseur. Depending on whether it is the marijuana version or the hemp version, the effects of this strain can vary. Evolutions cauldron: duplication, deletion, and rearrangement in the mouse and human genomes. It was beautifully grown and submitted by the Uruguayan growers and founders of El Limbo Cannabis Club; a cannabis club legally registered in IRCCA . Altschul S F, Gish W, Miller W, Myers E W, Lipman D J. Andersen E C, Gerke J P, Shapiro J A, Crissman J R, Ghosh R et al. To determine haplotype representation of the 61 divergent regions in other wild strains, we exploited the MPS sequence reads acquired previously (Thompson et al. Abstract. G3 (Bethesda) 4: 18131823. This strain can contain a moderate THC index of up to 15% and generates truly enjoyable balanced highs of a creative, euphoric, laughing, energetic, and happy nature. Cao J, Schneeberger K, Ossowski S, Gunther T, Bender S et al. . 2010; Vinuela et al. Insomnia . Kona Gold is a legendary, totally devastating Hawaiian Sativa from the Kailua-Kona side of the Big Island of Hawaii! This strain has the peculiarity of presenting small indices of CBD of up to 0.5% and CBN of up to 0.6%, which contribute synergistically to the final effects that this strain delivers. . The segments are scattered across all six chromosomes, range in size from 8 to >162 kb and show 215.8% sequence divergence from N2 without an obvious correlation between size and divergence (Figure 5). Royal Hawaiian is a strain that results from crossing Sativa and Indica strains native to the Hawaiian Islands. Natural variation in a neuropeptide Y receptor homolog modifies social behavior and food response in, Molecular hyperdiversity defines populations of the nematode, A genome-wide library of CB4856/N2 introgression lines of, Multiple reference genomes and transcriptomes for, Natural variation in a chloride channel subunit confers avermectin resistance in. The argument, 'pot wasn't as potent back then' generally refers to a time before Maui Waui was developed. All strains from Hawaii or called hawaiian, were from somewhere else. However, unlike BLASTZ, LASTZ can derive its own suitable tuning parameters from the sequences themselves. Its THC levels can reach up to 17%, and it can present CBG levels of up to 1%. This buds effects are famous for being lightheaded, relaxing but energetic at the same time, and for inducing creative waves that will put you in a perfect mood to enjoy the sun or have a good time with your friends. . The LASTZ alignments were performed with the chain option and lav output format. 71 Hawaii Strains Our work also emphasizes the importance of going beyond simple alignment of reads to a reference genome when assessing differences between genomes. The single case that did not agree was in one of the divergent regions. Hawaiian Breeze can have THC levels of up to 19% but is also available in a high CBD hemp version containing up to 19.8% CBD. These regions were also flagged with tools designed to detect alignment irregularities, such as REAPR and ALE (Clark et al. Alien Genetics' Hawaiian Ice is a THC dominant variety and is/was never available as feminized seeds. The Hawaiian strain (CB4856) of Caenorhabditis elegans is one of the most divergent from the canonical laboratory strain N2 and has been widely used in developmental, population, and evolutionary studies. A total of 22,261 transcripts were predicted, which were composed of 126,518 different exons. Hawaiian Cookies is an ideal strain for any time of the day, and it will allow you to tackle any list of pending activities feeling motivated with a cerebral high that will lift you. Open reading frames also exist in CB4856 for the final three exons of F47H4.2 and are partially incorporated into a second Genefinder model (Figure S9). The result of crossing the Hawaiian Snow and GSC or Girl Scout Cookies strains, the Hawaiian Cookies strain is one of a kind. Hawaiian reads from available RNA-seq projects (313,124,440 reads) (Stoeckius et al. The consistency between the integrated assembly and the JR-contigs suggests that many of the differences with the fosmid end sequences could be due to strain differences or from errors in the Sanger reads. To accomplish this goal, we took advantage of several very deep coverage MPS data sets for the Hawaiian genome, a de novo assembly program (Chu et al. elegans isolates). The mutational events (SNVs and indels, counting each indel as a single event) per aligned bases (percentage divergence) are plotted for each region against the length of the region in N2. (A) Alignment of 100-bp paired-end reads from the CB4856 genome to the N2 genome. 2007; Maydan et al. These effects can be of great help in helping some patients cope with insomnia, stress, and even depression. Our findings also have broader implications for studies comparing genome sequences to reference sequences using short reads. Jordan of the Islands' Blue Hawaiian is a THC dominant variety and is/was never available as feminized seeds. Anxiety calming energizing Hawaiian Sativa is a pure sativa. These regions of poor coverage persisted with different aligners that handle repeats differentlyphaster, which places all reads that match equally well at multiple copies of identical repeats at the first copy (P. Green, personal communication), and bwa or smalt (http://sanger.ac.uk/resources/software/smalt/), which distributes the matching reads randomly among the copies. The results reveal >60 regions with haplotypes that are substantially divergent from N2. However, Hawaiian Express will always provide you with lifting and energizing effects due to its Sativa nature. Some are extremely divergent and could represent overlapping deletions of an ancestral sequence, leaving unrelated sequences in the same place in the two present-day genomes. Lineage: Forum Cookies x High school Sweetheart. 2013), end sequences from a fosmid library for the Hawaiian genome, recently released RNA-seq data, and low-coverage genome sequence data from 49 RILs (Li et al. A. Thompson, and R. H. Waterston, unpublished results). The C. elegans N2 genome, version WS230 (note that while annotations changed, the N2 genome sequence remained identical from WS215 through WS234), was aligned against the Hawaiian genome using LASTZ [version 1.02.00; O = 400, E = 30, K = 3000, L = 3000, M = 0; (Harris 2007)]. As the hours go by, it will transform into a feeling of complete relaxation away from any feeling of being attached to the sofa or of bodily sedation. Hawaiian Dream is a perfectly balanced hybrid strain with slightly Sativa leaning effects. Also called Hawaiian Purple Kush, it is a delicious Indica-dominant hybrid strain with an 80/20 balance that will hit you with overwhelming waves of euphoria and incredible spikes of creativity. 2007; Ghosh et al. Here you find all cannabis varieties beginning or ending with "Hawaii"! These buds come loaded with a notorious 15 to 25% THC content that will make you feel its long-lasting effects after the first few hits. The sequence of these highly divergent regions and Hawaiian specific sequences must be determined to interpret more fully any genotypic and phenotypic differences between the Hawaiian and N2 strains. Hawaiian Heirloom Genetics - Dogwalker OG x Papaya (F) $ 150.00; Hawaiian Heirloom Genetics - Papa Petro (F) $ 150.00; Hawaiian Heirloom Genetics - Papa Le Pew (F) $ 150.00; Hawaiian Heirloom Genetics - Grease Monkey x Papaya (F) $ 150.00; Hawaiian Heirloom Genetics - Papa Melonz (F) $ 150.00; Hawaiian Heirloom Genetics - Citrilla . (D) The boundary of a divergent region (left) with a less divergent region of the genome is shown. To confirm that the JR contigs were properly placed within the genome, we examined coverage metrics for each contig across a collection of 60 introgression lines and 49 RILs. Only alignments to the same chromosome in N2 and Hawaiian and on the same strand were retained. Depression calming energizing low THC high THC Hawaiian Haze is a sativa marijuana strain that produces some. Assembler for de novo assembly of large genomes. van der Velde K J, de Haan M, Zych K, Arends D, Snoek L B et al. 2013), the SNV calls based on the new reference (SNVs), indels based on the new reference (Indels), and regions that failed to align with sequence present in the N2 reference (Unaligned). Talkative . Hawaiian Mango combines the deep Kush body effects with the revitalizing clear-head effects of Hawaiian strains, which can be ideal for treating poor appetite, moderate pain, insomnia, and symptoms of anxiety and depression. 2002). As a result, multiple regions of the Hawaiian genome remain missing or poorly defined. 2013). Creative Negatives: Paranoid . For example, among the 32 copies of Tc1 in the N2 reference, only 19 were detected in the CB4856 genome (Table S4). Its flavors are a mix between pineapple, peach, and lemon with some herbal and diesel notes. When there were gaps in either the N2 or Hawaiian genome between the major alignments, they were annotated as indels. As rapidly improving DNA sequencing technology has simplified both the detection and the cataloging of variation, major efforts have been undertaken to describe variation and then analyze quantitative traits in wild isolates of various model organisms, including Caenorhabditis elegans, Saccharomyces cerevisiae, Drosophila, and Arabidopsis (Schacherer et al. 2012; http://www.1001genomes.org). The high level of divergence between the two haplotypes of N2 and CB4856 in these regions indicates an ancient origin. Nonetheless, 517 Legend Seed Co suggests that Hawaiian Pancake is the result of crossing the Blueberry Pancake and Rambutan strains, so this relatively new rarity on the cannabis market shares genetics with the world-renowned, and previously mentioned, Hawaiian Maui Wowie. 2008; Rockman et al. This strain is famous for reaching peculiarly high CBD levels of up to 12% and THC concentrations of between 4 and 12%. Because of the shorter seasons, they were bred to finish fast in the seasons. After a few months of study and development, they managed to find the smells and effects they were looking for in a genuinely unique bud called Hawaiian Pepper. 2010), we find that more than one-third of their deletion calls fall within these divergent regions, often extending across most of the region. All the CB4856 chromosomes are smaller than their N2 counterparts with II and V showing the greatest reductions at 2.57 and 3.54%, respectively (Table 2). Lineage Breeding designs for recombinant inbred advanced intercross lines. The resulting output contains a single set of ordered alignments in the N2 genome followed by additional fill in alignments. Well, that's exactly what Hawaiian cannabis strains can create. In toto from the indels and reciprocal indels, N2 has 3.3 Mb of sequence not represented in CB4856, and CB4856 has 1.4 Mb of sequence not present in N2 (Table 3). Snoek, L. B., H. E. Orbidans, J. J. Stastna, A. Aartse, M. Rodriguez et al., 2014Widespread genomic incompatibilities in Caenorhabditis elegans. Hawaiian Purple Kush is a delicious strain that combines all the therapeutic magic and deep relaxation that the popular Purple Kush strain provides, along with the brain-lifting and lifting effects of the Hawaiian strain. The resulting CB4856 reference sequence extends and refines the scope of the variation between N2 and CB4856 from prior studies. It shows multiple phenotypic differences with N2, including production of a copulatory plug, aggregation behavior, a lack of temperature-size dependence, growth rate, fecundity, RNA interference insensitivity by feeding and drug resistance (de Bono and Bargmann 1998; Kammenga et al. Of those, 8380 were in a divergent region, and 8123 of those were confirmed (96.9%). . Hawaiian Big Bud is an Indica-dominant hybrid strain with a balance of 85/15 that can feature high THC levels of up to 21%. In addition, 816 segments with sequence in both genomes failed to align with LASTZ and also failed to produce consistent alignments with different SmithWaterman alignment implementations. Inspection of the alignments and ALE score patterns across the region suggested that the strain had intermediate divergence across the region, where some blocks of a region resembled the N2 haplotype and others resembled CB4856 (Figure S4B). Sites that differed by base substitution and insertion and deletion were recognized, and the N2 genome was altered at those sites. Energetic Negatives: Dizzy . Added to this, the Indica side of this hybrid strain will give you a feeling of calm and well-being that will automatically eliminate any physical pain. Regardless of the origin, haplotypes for only a few regions have persisted, perhaps maintained by balancing selection. Continue Reading Below Advertise on Wikileaf Hawaiian Diesel struts very well, filling its position as one of the best Hawaiian strains currently available on the cannabis market. RNA-seq biases introduced by mapping to the N2 reference may also distort any signal. Hawaiian Elephant, also known as Old Elephant, is a strain of pakall (cannabis) Sativa that became popular in the 1960s and 1970s. The result of this cross is an exquisite Indica-dominant strain with an 80/20 balance that can reach THC levels of between 15% to 23%. Jordan of the Islands' Blue Hawaiian Description To assess the alignment quality, we calculated ALE scores using the N2 and CB4856 reads aligned against each other as controls. These results suggest that, rather than containing an inactivated gene, the CB4856 region encodes a homologous protein. Curiously, in comparing our results with prior studies using array CGH (Maydan et al. . . The Hawaiian strain of Caenorhabditis elegans is one of the most divergent from the canonical laboratory strain N2 and has been widely used in developmental, population, and evolutionary studies.To enhance the utility of the strain, we have generated a draft sequence of the CB4856 genome, exploiting a variety of resources and strategies.As previously reported, the density of SNVs . But the larger deletions (>5 bases) are slightly more numerous in CB4856 (12,181 vs. 9803), and the overall the number of bases deleted in CB4856 is much larger (2.2 Mb of ancestral genome lost in CB4856 vs. 0.92 Mb in N2). To correct these areas, individual reads were assembled de novo with the JR-Assembler and aligned against the modified reference. It can be an ideal strain for treating conditions like anxiety, stress, depression, fatigue, and insomnia. This cross between Sunset Sherbet and Sour Apple IBL has a great reputation for its incredible terpene profile and set of mellow effects. The autosomal divergent regions include the peel-1 zeel-1 region and the glc-1 gene, both of which had been previously reported as having an elevated sequence divergence (Seidel et al. Next, we looked at the distribution of the SNVs and indels across and within the chromosomes. In addition to the indels, the 816 reciprocal insertion/deletion events show a similar trend of greater loss of sequence in the CB4856 genome, with these segments containing 458,526 bases in CB4856 and 1,109,331 bases in N2. So addition of new genetics is very common. A certain mysterious brilliance surrounds the genetics of these tropical beauties. 5 Laboratory of Systems Biology and Genetics, Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fdrale de . Perhaps these divergent regions are a remnant of the variation present at the conversion to a hermaphroditic species, with all 61 regions maintained by balancing selection since the origin of the species. 2012; Pollard and Rockman 2013; Andersen et al. . Headache Helps with: Stress . . In addition to coverage, the tracks show the SNV calls (MMP SNVs) reported in (Thompson et al. They also contain the bulk of genes for large, rapidly evolving gene families. Paranoid . Gene models for each region are shown below. . Depression . You can find Hawaiian Haze available in the market both in its CBD version that can have CBD levels of up to 14% and in its high THC version that can climb up to 18%. In that case, Hawaiian Golden Pineapple may be an ideal option for you. Supporting information is available online at www.genetics.org/lookup/suppl/doi:10.1534/genetics.115.175950/-/DC1. But, in addition, inspection of the deep WGS coverage revealed some regions of the genome that apparently were so divergent that aligned reads were sparse to absent (D. Spencer, O. Ghosh R, Andersen E C, Shapiro J A, Gerke J P, Kruglyak L. Gerstein M B, Rozowsky J, Yan K K, Wang D, Cheng C et al. Most of the strains have unique combinations of sequences; however, JU1171, MY2, and MY14 all share the same pattern (both MY2 and MY14 were isolated from Munster, Germany; share 96% of SNV calls and share 96% with JU1171, isolated in Chile; they likely represent a single isotype), and ED3057 and ED3072 are similar (the latter two were both isolated in Kenya and share 97% of their SNV calls and also likely represent a single isotype). Just as the N2 reference has been improved through continuous community input, we expect users will provide improvements here. This strain results from a cross between the UK Cheese and Lavender strains and exudes a mixture of aromas that honor its genetics with some earthy, herbal notes and some hints of cheese. This marvel of cannabis offers its users an exciting scent profile with tones of diesel and earth and enjoyable effects with the ability to relax the muscles and immediately cleanse any pain or stress from your day. Hungry . 2012). Regardless of the nature of the original event(s), balancing selection is likely to be playing a role in the maintenance of these regions across the globe. As a result, they had not been removed in our iterative alignment and correction procedure. Our cultivation crew loves pheno-hunting for new strains. Pain calming. 2012). Recombinational landscape and population genomics of, Selection at linked sites shapes heritable phenotypic variation in, Comprehensive polymorphism survey elucidates population structure of, Reference-guided assembly of four diverse, A novel sperm-delivered toxin causes late-stage embryo lethality and transmission ratio distortion in, WormQTL: public archive and analysis web portal for natural variation data in, High genetic diversity in the chemoreceptor superfamily of. These effects promote focus and creativity while generating a state of physical and mental relaxation that is perfect for treating chronic stress and pain, nausea, cravings, and depression. The THC index of Hawaiian Delight is around 20%, and due to its Indica nature, it is recommended for the end of the day or a time when you can fully enjoy the relaxation that this strain will provide you. These cannabinoids can help deal with chronic pain and muscle spasms and work as an anti-inflammatory. Seidel H S, Ailion M, Li J, van Oudenaarden A, Rockman M V et al. This process allowed extension of sequence into regions of high divergence, closed large deletions, and built sequence into insertions (Figure 1 and Figure 2). It contains a delicious combination of tropical flavors and smells with hints of berries and cherries and moderate THC levels. Hawaiian Pineapple can work effectively to deal with conditions like epilepsy, glaucoma, fatigue, and PTSD. 2012). . The genes altered by the LOF variants are disproportionately composed of members of large, rapidly evolving gene families, including the math, bath (btb-math), clec (c-lectin), fbx (F-box), and seven-transmembrane serpentine receptor genes. THC/CBD Content This powerhouse carries an impressive 25% THC content in its leaves. In 415 of the 416 cases, or less than half a percent of the cases, the base called in the JR-Assembler contig agreed with the Hawaiian SNV call. Hawaiian Pepper is a truly unique strain initially created by members of a musical group called "Pepper," hence its name. MPS sequence reads from each of the 39 wild isolates previously studied (Thompson et al. Hawaiian Butterscotch generates long-lasting effects with nice spikes of creativity and happiness included. The same large, rapidly evolving gene families are also heavily represented in the divergent regions. Whether you are miles away from the tropics or the nearest beach, the delicious Hawaiian strains can certainly recreate a heavenly beach environment wherever you are. DNA from the C. elegans CB4856 strain was extracted using the Qiagen Blood and Tissue kit and quantified using a Qubit 2.0 broad-range kit. Still, many users rate this strain as a complete creeper since its effects take a reasonable longer time to hit than many of the better-known hybrid strains. . Specific methods for each of these steps are presented in the Supporting Information. This rare and unique strain of medical cannabis, as the name suggests, is like a tropical dream filled with happiness, laughter, and a refreshing sense of tranquility full of vitality and creative energy. Both high in THC and known for its head-hitting effects, the great well-being properties found in these strains were passed down to Hawaiian Breeze, which was specially bred to deliver the best of cannabis for those who want to hang lucid. Hawaiian Gold is a Sativa-dominant hybrid strain resulting from the crossing of Pineapple and Super Lemon Haze strains. 2011), we first aligned the random genomic reads to the N2 reference genome, identified SNVs and indels, modified the N2 reference accordingly, and realigned the reads, repeating the process 19 times to create a first version of the Hawaiian genome (20 cycles total) (Figure 1; Supporting Information, Figure S1; Table S1). Peach Ringz mixes light and dark green with dapples of purple and red pistils. Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Like F47H4.2, other genes that appear to have suffered a LOF variant in CB4856 also have Genefinder models in syntenic regions that are often supported by RNA-seq data and may similarly encode full-length proteins. Hawaiian Trinity produces buds that can contain an impressive 21% THC that can generate a physical rush effect that subsides into a sedative-physical high as it unfolds. However, inspection of the variants in these genes in comparison with WormBase models and the extensive modENCODE RNA-seq data (Gerstein et al. Applying the same methods to the 61 regions described here gives an overall estimate of dS (average number of nucleotide differences between sequences per synonymous site) of 0.12 and similar estimate of 6.7 106 generations. Hawaiian Sunrise can contain 15% to 25% THC and up to 11% CBD, which generates truly unique effects on users of this strain in conjunction with its terpene profile. Pain calming energizing low THC high THC Hawaiian. For full access to this pdf, sign in to an existing account, or purchase an annual subscription. Each N2 gene was aligned to the corresponding Hawaiian chromosome. One geneF47H4.2stood out because of the multiple changes reported from the LASTZ alignments, including 691 SNVs across the two isoforms that, considered together, result in 511-amino-acid substitutions, 1 nonsense mutation, and 13 frameshifting indels. However, some isolates appear to have regions that are divergent from both N2 and CB4856 and in segments outside the divergent regions some strains show divergence from both N2 and CB4856. ThugPug Genetics' Hawaiian Unicorn is a THC dominant variety and is/was never available as feminized seeds. 2012). These buds, when smoked, emanate a dense sweet smoke with sour flavors and smells of pineapple, skunk, and spicy overtones. Chu T C, Lu C H, Liu T, Lee G C, Li W H et al. This top-shelf hybrid strain is the result of crossing the Blueberry and Hawaiian Sativa strains and can be an excellent option to deal with headaches, migraines, anxiety, stress, and depression. Despite these multiple variants, a Genefinder model in the region of CB4856 predicts a protein of 628 amino acids with its seven exons having open reading frames similar in length to those in N2 and with six of those having splice junctions in precisely equivalent places (Figure S9). Only problems now, is most of the new stuff doesn . This strain can reach THC levels of between 19% to 25% and is famous for causing giggling, euphoric, energetic, and happy highs while allowing you to stay focused. As the high progress, it transforms into a state of physical and mental relaxation. A result of crossing the Hawaiian and Pink Runtz strains, the popular Hawaiian Runtz is already famous for itself. Alignment of 42,261 Sanger reads from fosmid ends and plasmids (Table 1) also confirmed the substitutions and indels introduced in new CB4856 reference (Supporting Information). Depression calming. Ogre Breath is GMO x Meat Breath and was made in 2020. The Hawaiian strain (CB4856) of Caenorhabditis elegans is one of the most divergent from the canonical laboratory strain N2 and has been widely used in developmental, population, and evolutionary studies. Hunt M, Kikuchi T, Sanders M, Newbold C, Berriman M et al. Using a strategy similar to that employed in the analysis of different Arabidopsis accessions (Gan et al. It is an Indica-dominant hybrid strain with a balance of 70/30 that can reach THC levels of between 15% to 20%. However, we found evidence for Tc1 copies in the CB4856 not present in N2. . Its popularity was because its 13 - 19% THC content was much . Sativa Strain Reviews March 30, 2023 You may have heard of the Dutch Hawaiian strain. This bud results from the crossing of the Hawaiian and Cotton Candy Kush strains and can present THC levels of up to 17%. The JR contigs aligned against N2 showed base-pair disagreements consistent with the called SNVs in most regions of the genome, i.e., those that had good coverage with the CB4856 sequence reads.

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